At least five teams either fielded an ineligible player or had one on their gameday roster during the first or second rounds of the tournament. However, only two of the teams were discovered in time to file a protest.
Kraze United
2016 US Open Cup Round 1: The Villages stun Kraze United in stoppage time in battle of re-branded teams
The Villages SC (PDL) of The Villages, Fla. hosted Kraze United (NPSL) of Orlando at The Villages Polo Field, stunning their guests after 91 minutes of scoreless soccer on a last-minute goal to move on to the second round.