The 2010 USASA Region I Final tournament will be the largest in professional era history. It will feature 16 teams from eight different member associations and the National Premier Soccer League. Eight of these clubs have participated in previous US Open Cup tournaments, with five titles being shared between two of them. The tournament begins with first round action on April 11, and the 2010 US Open Cup participants will be determined by May 23 after the conclusion of the semifinals. The clubs (and previous USOC appearances in the pro era) are listed below:
State champions
Athletic Club – Western Pennsylvania
Club Atletico Virginia – DC/Virginia
Danbury United – Connecticut (2007)
Emigrantes das Ilhas – Massachusetts (2009)
Go-Soccer FC – New Jersey
New York Pancyprian Freedoms – Eastern New York
Phoenix SC – Eastern Pennsylvania
TBD – Maryland (Jilly’s Bar & Grill/Charm City FC winner)
Other entrants
Boston Olympiakos – Massachusetts (2008)
Brooklyn Italians – NPSL Northeast
Dulles Sportsplex Aegean Hawks – DC/Virginia (2007, 2009)
Erie Admirals – NPSL Northeast
Fenerbahce USA – Eastern New York
Olympia Stamford – Connecticut (2001)
United German Hungarian Majors – Eastern Pennsylvania (1999)
TBD – Maryland (Jilly’s Bar & Grill/Charm City FC loser)
Bracket
| First Round (April 11) | Quarterfinals (May 2) | Semifinals (May 23) | Final (June 27) | |||
| Danbury United (CT) | ||||||
| Boston Olympiakos (MA) | ||||||
| Fenerbahce USA (E NY) | ||||||
| Erie Admirals (NPSL) | ||||||
| Go-Soccer FC (NJ) | ||||||
| Olympia Stamford (CT) | ||||||
| Brooklyn Italians (NPSL) | ||||||
| Emigrantes das Ilhas (MA) | ||||||
| DS Aegean Hawks (DC/VA) | ||||||
| Maryland #2 | ||||||
| NY Pancyprian Freedoms (E NY) | ||||||
| Phoenix SC (E PA) | ||||||
| Maryland #1 | ||||||
| Athletic Club (W PA) | ||||||
| UGH Majors (E PA) | ||||||
| Club Atletico (DC/VA) |