Click on the scores below for match reports
* 2013 US Open Cup bracket
* 2013 US Open Cup goalscoring leaders
* The First Cup series (1913/14)
| PRELIM. ROUND |
AWAY TEAM | SCORE | HOME TEAM |
| May 7 | Georgia Revolution (NPSL) | 1:1 (5:3 PK) | Colorado Rovers (USSSA) |
| May 7 | FC Hasental (NPSL) | 5:3 | Fresno Fuego Future (USCS) |
| FIRST ROUND | AWAY TEAM | SCORE | HOME TEAM |
| May 14 | Real Colorado Foxes (PDL) | 3:4 | Georgia Revolution (NPSL) |
| May 14 | FC Lehigh Valley United (NPSL) | 0:2 | Reading United (PDL) |
| May 14 | VSI Tampa Bay FC (USL Pro) | 1:1 (5:3 PK) | Orlando City U23s (PDL) |
| May 14 | Chattanooga FC (NPSL) | 4:4 (1:4 PK) | Carolina Dynamo (PDL) |
| May 14 | RWB Adria (USASA) | 1:1 (4:5 PK) | Pittsburgh Riverhounds (USL Pro) |
| May 14 | River City Rovers (PDL) | 0:3 | Dayton Dutch Lions (USL Pro) |
| May 14 | New York Red Bulls U23s (NPSL) | 0:2 | Ocean City Nor’easters (PDL) |
| May 14 | Ocala Stampede (PDL) | 4:2 | Red Force (USASA) |
| May 14 | Dearborn Stars SC (USASA) | 2:0 | Michigan Bucks (PDL) |
| May 14 | Mass Premier Soccer (USASA) | 0:2 | GPS Portland Phoenix (PDL) |
| May 14 | Icon FC (USASA) | 4:1 | Brooklyn Italians (NPSL) |
| May 14 | North Texas Rayados (USASA) | 0:3 | Austin Aztex (PDL) |
| May 14 | PSA Elite (USASA) | 0:2 | Laredo Heat (PDL) |
| May 14 | FC Hasental (NPSL) | 2:3 | Ventura County Fusion (PDL) |
| May 14 | Doxa Italia (USASA) | 1:5 | Seattle Sounders U23s (PDL) |
| May 14 | Phoenix FC (USL Pro) | 1:2 | FC Tucson (PDL) |
| May 14 | Sacramento Gold (NPSL) | 2:3 | Portland Timbers U23s (PDL) |
| May 15 | Madison 56ers (NPSL) | 0:1 | Des Moines Menace (PDL) |
| SECOND ROUND | AWAY TEAM | SCORE | HOME TEAM |
| May 21 | Icon FC (USASA) | 1:4 | Richmond Kickers (USL Pro) |
| May 21 | Dearborn Stars SC (USASA) | 1:4 (AET) | Dayton Dutch Lions (USL Pro) |
| May 21 | Orlando City SC (USL Pro) | 2:1 | Ocala Stampede (PDL) |
| May 21 | Seattle Sounders U23s (PDL) | 0:3 | Charlotte Eagles (USL Pro) |
| May 21 | Atlanta Silverbacks (NASL) | 3:2 | Georgia Revolution (NPSL) |
| May 21 | Carolina Dynamo (PDL) | 1:3 | Carolina RailHawks (NASL) |
| May 21 | H’burg City Islanders (USL Pro) | 0:1 | Reading United (PDL) |
| May 21 | Tampa Bay Rowdies (NASL) | 2:1 | VSI Tampa Bay FC (USL Pro) |
| May 21 | Pittsburgh Riverhounds (USL Pro) | 0:1 | Ocean City Nor’easters (PDL) |
| May 21 | Laredo Heat (PDL) | 1:1 (6:7 PK) | Fort Lauderdale Strikers (NASL) |
| May 21 | GPS Portland Phoenix (PDL) | 0:1 | Rochester Rhinos (USL Pro) |
| May 21 | Des Moines Menace (PDL) | 1:0 | Minnesota United FC (NASL) |
| May 21 | FC Tucson (PDL) | 2:2 (4:3 PK) | San Antonio Scorpions (NASL) |
| May 21 | Wilm. Hammerheads (USL Pro) | 2:0 | Austin Aztex (PDL) |
| May 21 | Ventura County Fusion (PDL) | 1:5 | Los Angeles Blues (USL Pro) |
| May 21 | Charleston Battery (USL Pro) | 1:0 | Portland Timbers U23s (PDL) |
| THIRD ROUND | AWAY TEAM | SCORE | HOME TEAM |
| May 28 | DC United (MLS) | 0:0 (4:2 PK) | Richmond Kickers (USL Pro) |
| May 28 | San Jose Earthquakes (MLS) | 0:1 | Charleston Battery (USL Pro) |
| May 28 | Colorado Rapids (MLS) | 1:3 | Orlando City SC (USL Pro) |
| May 28 | FC Dallas (MLS) | 2:0 | Ft. Lauderdale Strikers (NASL) |
| May 28 | New England Revolution (MLS) | 5:1 | Rochester Rhinos (USL Pro) |
| May 28 | Ocean City Nor’easters (PDL) | 1:2 | Philadelphia Union (MLS) |
| May 28 | Des Moines Menace (PDL) | 0:2 | Sporting Kansas City (MLS) |
| May 28 | Atlanta Silverbacks (NASL) | 2:3 (AET) | Real Salt Lake (MLS) |
| May 28 | Chivas USA (MLS) | 2:1 | Los Angeles Blues (USL Pro) |
| May 29 | Chicago Fire (MLS) | 2:0 | Charlotte Eagles (USL Pro) |
| May 29 | Los Angeles Galaxy (MLS) | 0:2 | Carolina RailHawks (NASL) |
| May 29 | Dayton Dutch Lions (USL Pro) | 1:2 | Columbus Crew (MLS) |
| May 29 | Seattle Sounders (MLS) | 0:1 | Tampa Bay Rowdies (NASL) |
| May 29 | Reading United (PDL) | 0:2 | New York Red Bulls (MLS) |
| May 29 | FC Tucson (PDL) | 0:2 | Houston Dynamo (MLS) |
| May 29 | Wilm. Hammerheads (USL Pro) | 1:5 | Portland Timbers (MLS) |
| FOURTH ROUND | AWAY TEAM | SCORE | HOME TEAM |
| June 12 | Philadelphia Union (MLS) | 1:3 | DC United (MLS) |
| June 12 | Chivas USA (MLS) | 1:3 (AET) | Carolina RailHawks (NASL) |
| June 12 | New York Red Bulls (MLS) | 2:4 | New England Revolution (MLS) |
| June 12 | Orlando City SC (USL Pro) | 1:0 | Sporting Kansas City (MLS) |
| June 12 | Houston Dynamo (MLS) | 0:3 | FC Dallas (MLS) |
| June 12 | Charleston Battery (USL Pro) | 2:5 (AET) | Real Salt Lake (MLS) |
| June 12 | Tampa Bay Rowdies (NASL) | 0:2 | Portland Timbers (MLS) |
| June 13 | Columbus Crew (MLS) | 1:2 | Chicago Fire (MLS) |
| QUARTERFINALS | AWAY TEAM | SCORE | HOME TEAM |
| June 26 | New England Revolution (MLS) | 1:3 | DC United (MLS) |
| June 26 | Orlando City SC (USL Pro) | 1:5 | Chicago Fire (MLS) |
| June 26 | Portland Timbers (MLS) | 3:2 | FC Dallas (MLS) |
| June 26 | Carolina RailHawks (NASL) | 0:3 | Real Salt Lake (MLS) |
| SEMIFINALS | AWAY TEAM | SCORE | HOME TEAM |
| August 7 | DC United (MLS) | 2:0 | Chicago Fire (MLS) |
| August 7 | Portland Timbers (MLS) | 1:2 | Real Salt Lake (MLS) |
| FINAL | AWAY TEAM | SCORE | HOME TEAM |
| Oct. 1 | DC United (MLS) | 1:0 | Real Salt Lake (MLS) |